Objective:To investigate the effect of interleukin-13(IL-13) on mucus secretion in vitro and the possible mechanism.Methods: HBE16 cells were serum-starved for 24 h and exposed to IL- 13 for 24 h. The level of MUC5AC was detected using ELISA. The phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinase 1/2 (JNK1/2) were also examined also. The cells were pretreated with SP600125 for 1 h and then the level of MUC5AC was measured. RT-PCR was performed to examine the mRNA level of STAT4 and STAT6. Electrophoretic mobility shift assays (EMSA) were performed to examine the DNA-binding activity of Forkhead box a2 (FOXA2). Results:IL-13 caused a significant increase of MUC5AC and p-JNK1/2, yet failed to affect the phosphorylation of ERK1/2. The expression of MUC5AC was attenuated after treatment with SP600125.A significant increase in STAT6 was observed in IL-13 group compared to that of the saline-treatment group, whereas the expression of STAT4 was not significantly affected. The DNA-binding activity of FOXA2 was down-regulated after exposed to IL-13. Conclusion:Our study suggestes that IL-13 down-regulates mucus secretion via JNK-STAT6-FOXA2 pathway in vitro.%目的:研究白介素13 (Interleukin-13,IL-13) 对气道上皮细胞粘液分泌的效应并探讨其作用机制.方法:HBE16细胞在无血清培养基中培养24小时后加入IL-13刺激24小时,ELISA检测粘蛋白(MUC)5AC的表达;Western检测磷酸化细胞外信号调节激酶1/2(Extracellular signal-regulated kinase 1/2,ERK1/2) 和磷酸化c-Jun氨基端激酶1/2 (c-Jun N-terminal kinase 1/2,JNK1/2 的表达;使用SP600125阻滞JNK信号通路后检测MUC5AC蛋白表达;RT-PCR 检测STAT4和STAT6的表达变化;EMSA检测通路下游核蛋白FOXA2的表达.结果:IL-13刺激24小时后MUC5AC和p-JNK1/2表达升高,而p-ERK1/2表达无显著变化;使用SP600125阻断JNK通路表达后,MUC5AC表达减弱;IL-13刺激后STAT4表达无显著变化,STAT6表达显著升高,FOXA2表达显著降低.结论:IL-13通过JNK-STAT6-FOXA2通路调控粘液分泌.
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