F-Z液与UW液保存效果的比较研究

摘要

目的 比较F-Z液与Uw液在细胞水平的保存效果.方法 1.测定内皮细胞经保存-复温后上清中乳酸脱氢酶(LDH)浓度,比较保存一复温后损伤;2.以MTT法比较保存-复温后的内皮细胞活力.结果 1.保存时间24 h内,UW液组上清中乳酸脱氧酶(LDH)浓度与F-Z液组无明显差别;2.保存时间48 h内,UW液组MTT结果与F-Z液组无明显差别.结论 在细胞水平,于减轻保存-复温后损伤和维持被保存细胞活力两个方面,F-Z液均达到UW液相似的保存效果,因而具进一步开发研究价值.%Objective To compare F-Z solution with UW solution in preserving efficacy at cell level. Methods The LDH content in supernatant of endothelial cells after preservation-rewarming to determine the injury after preservation-rewarming. MTT was used to determine the activity of endo-thelial cells after preservation-rewarming. Results After being preserved for 24 h, the LDH content in supernatant of endothelial cells in UW group was not significantly different from that in F-Z group.After preservation for 48 h, there was no marked difference in the activity of endothelial cells between the 2 groups. Conclusion F-Z solution and UW solution have the same effect in reducing injury and maintaining activity of endothelial cells after preservation-rewarming at cell level.