目的 比较软组织内热针与其他微创疗法对大鼠骨骼肌、腓肠神经慢性损伤后血管内皮生长因子(VEGF)及内源性碱性成纤维细胞生长因子(BFGF)活性的影响.方法 将75只雄性Wistar大鼠随机分为损伤对照组、内热针组、银质针加热组、银质针未加热组、小针刀组、射频组各12只及正常对照组3只.各治疗组在治疗3、7、14、28 d时分别处死实验大鼠3只,对损伤部位的组织进行VEGF、BFGF测定.结果 (1)治疗3d后腓肠肌肌肉肌腱接头处组织和腓肠神经中VEGF和BFGF活性升高,且各治疗组高于损伤对照组(P＜0.01),内热针组、银质针加热组高于其他组(P＜0.05);(2)7 d和14d,内热针组、银质针加热组VEGF、BFGF明显高于其他治疗组(P＜0.01),且内热针组高于银质针加热组(P＜0.05); (3)28 d,各治疗组VEGF、BFGF低于损伤对照组(P＜0.01),内热针组、银质针加热组低于银质针未加热组、小针刀组、射频组(P＜0.01),且内热针组低于银质针加热组(P＜0.05).结论 软组织内热针和银质针加热疗法可以明显提高大鼠骨骼肌和神经慢性损伤组织中VEGF、BFGF活性,促进组织修复,增强机体恢复能力.软组织内热针治疗效果优于银质针可能与其针刺内热稳定性高有关.%Objective To observe the effects of the internally heated needle and other micro-trauma therapy on VEGF and BFGF in the repaired tissue(gastrocnemius muscle tendon junction and sural nerve)of rats with chronic injuries. Methods seventy-five healthy male Wistar rats were randomly divided into injury control group (n=12),internal heated needle group (n=12),silver needle with heat group (n=12), silver needle without heal group (n=12) .small needle knife group (n=12),radio frequency therapy group (n=12)and normal control group (n=3). The level of VEGF and bFGF in the repaired tissue were measured at day3,7,14 and 28. Results The bFGF and VEGF activity in all therapy groups are higher than that in the injury control group on the day3,7rnand 14 (P<0.01). On day 3,the internal heated needle group and the silver needle with heat group showed higher results than others (P<0.01)jday 7and day 14's levels of VEGF and bFCF were the highest (P<0.01);on day 28,the statistics of the therapy groups decreased compared to those of the control group (P<0.01) ,and the statistics of the internal heated needle group and the silver needle with heat group were lower than those of others (P<0.01). Conclusions The internal healed needle and the silver needle with heat treatments can increase the bFGF and VEGF activity in rats after their chronic skeletal muscle and nerve injury. The former is relatively better than the latter in therapeutic effect.
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