益气化痰祛瘀方对冠心病模型大鼠PAI-1、t-PA表达的影响

摘要

目的 观察益气化痰祛瘀方对冠心病大鼠心肌及主动脉组织PAI-1、t-PA表达的影响及作用机制.方法 采用高脂饲料、脑垂体后叶素腹腔注射建立大鼠冠心病模型,分为正常组、模型组、复方丹参滴丸对照组、益气化痰祛瘀方高、低剂量组,采用免疫组化法检测大鼠心肌和主动脉组织PAI-1、t-PA的表达.结果 模型组大鼠心肌和主动脉组织PAI-1表达增强,t-PA表达减弱(P＜0.01);给药后各用药组PAI-1的表达减弱,t-PA的表达增强(P＜0.01);高、低剂量组均优于对照组(P＜0.05或P＜0.01),高、低剂量组间无明显差异(P＞0.05).结论 益气化痰祛瘀方可通过调节心肌和主动脉组织PAI-1、t-PA的表达,以达到治疗冠心病的目的.%Objective To observe the effects of Prescription Yiqi Huatan Quyn on the PAI-1 and t-PA expression in the myocardial and aortic organization of the CHD rats and explore the possible mechanism of prescription Yiqi Huatan Quyu in the therapy of CHD. Methods The model of CHD rats was established by feeding high fat forage and injecting pituitrin in the abdominal cavity. Then these rats were divided into normal control, model control, compound danshen drop pill(control) ,high dose,low dose groups. Then the PAI-1 and t-PA expression in the myocardial and aortic organization of the all groups laboratory rats were measured by immunohistochemistry method. Results The PAI-1 expression in the myocardial and aortic organization of the model group were increased (P ＜ 0. 01 ). The t-PA expression in the myocardial and aortic organization of the model group were reduced ( P ＜0. 01 ). The PAI-1 expression reduced ( P ＜0. 01 ) and the t-PA expression increased (P ＜0. 01 ) of each treatment group. The high dose group and low dose group were more better than those of compound danshen drop pill control group( P ＜0. 05 or P ＜0. 01 ). But it had no obvious difference between high and low dose group ( P ＞ 0. 05 ). Conclusions Prescription Yiqi Huatan Quyu has the effect of adjusting PAI-1 and t-PA expression in the myocardial and aortic organization.