Objective To investigate HSV discharge in cervix and male urethra of different clinical samples from genital herpes (GH)by touchdown PCR. Methods One sample was taken from every genital herpes. Then clinical samples were identified by touchdown PCR. Results Among 38 GH patients, 32 samples were positive by touchdown PCR and identified HSV-E ;while 7 samples were positive by cell culture and identified HSV by PCR. The HSV positive rate of touchdown PCR was 78. 0% while cell culture was 14. 1%. There was statistical significance in the positive rate between two methods (x2 = 30. 56,P <0. 01). Conclusions Touchdown PCR would be the clinically preferable method than cell culture to detect HSV in different samples from GH.%目的 应用降落PCR法检测初发性和复发性生殖器疱疹患者尿道和宫颈HSV排放情况.方法 对每位初发性和复发性生殖器疱疹患者均取1个拭子,采用降落聚合酶链式反应(TD-PCR)对临床标本进行鉴定.结果 共检测38例生殖器疱疹患者,男性尿道口标本28个,初发性7个,复发性21个；女性宫颈管标本10个,初发性和复发性各5个.经降落PCR方法鉴定,均为HSV-Ⅱ.初发病例与复发病例的HSV-PCR阳性率分别为83％ (10/12)和46.2％ (12/26)(x2=4.66,P＜0.05),有显著性差异.结论 初发性和复发性生殖器疱疹患者尿道和宫颈HSV排放的情况不同,初发性生殖器疱疹的排毒率高于复发性生殖器疱疹.
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