Distribution of functional microorganisms and its significance for iron, sulphur, and nitrogen cycles in reservoir sediments

摘要

The biogeochemical cycles of sulphur(S),iron(Fe)and nitrogen(N)elements play a key role in the reservoir ecosystem.However,the spatial positioning and interrelationship of S,Fe and N cycles in the reservoir sediment profile have not been explored to a greater extent.Here,we measure the gradients of Fe^(2+),SO_(4)^(2-),NO_(3)^(-),NH_(4)^(+),DOC,TC and TN in the pore water of the sediment,and combining the vertical distribution of the functional microorganisms involved in S,Fe and N cyclings in the sediments to determine the redox stratification in the sediment.It is found that the geochemical gradient of S,Fe and N of the reservoir sedimentary column is mainly defined by the redox process involved in the related functional microorganisms.According to the type of electron acceptor,the sediment profile is divided into 3 redox intervals,namely aerobic respiration(0–10 cm),denitrification/iron reduction(10–28 cm)and sulfate reduction(28–32 cm).In the aerobic respiration zone,NH_(4)^(+)is oxidized by aerobic AOB to NO_(3)^(-)(0–5 cm),and Fe^(2+)is oxidized by microaerobic FeRB to Fe^(3+)(3–10 cm).In the denitrification/iron reduction zone,Acinetobacter and Pseudomonas,as the dominant NRB genera,may use nitrate as an electron acceptor to oxidize Fe^(2+)(11–16 cm).The dominant genera in SOB,such as Sulfururvum,Thiobacillus and Thioalkalispira,may use nitrate as an electron acceptor to oxidize sulfide,leading to SO_(4)^(2-)accumulation(14–24 cm).In the sulfate reduction zone,SO_(4)^(2-)is reduced by SRB.This study found that functional microorganisms forming comprehensive local ecological structures to adapt to changing geochemical conditions,and which would be potentially important for the degradation and preservation of C and the fate of many nutrients and contaminants in reservoirs.