浙南地区血液核酸集中化检测筛查数据回顾性分析

摘要

Objective Through a retrospective analysis of serology and nucleic acid screening data of unpaid blood donors in south Zhejiang region,the role of nucleic acid detection in the reduction of transfusion-related infectious diseases was discussed.Methods 179 369 unpaid blood donation in south Zhejiong province from Jan,2016 to Dec,2016 was chosen.Enzyme linked immunosorbent (ELISA) test for blood index of infectious hepatitis B virus surface antigen (HBsAg),hepatitis C virus antibody (antiHCV),human immunodeficiency virus antigen and antibody.At the same time the roche,haoyuan nucleic acid detection system were used for HBV-DNA,HCV-RNA,HIV-RNA in 6 or 8 doses mix samples three projects joint detection.NAT results of the serum with negative results in the serological tests were made statisticala analysis.Results A total of 259 ELISA-/NAT + samples were detected,HBV-DNA + 255 cases,HCV-RNA + 5 cases,one case of HBV-DNA and HCV-RNA +,with a positive rate of 0.14％.The analysis system of roche nucleic acid mix inspection rate of positive was 1.40％,and the split inspection rate of positive was 60.72％.The mix inspection positive rate of the haoyuan analysis system was 1.63％,and the split inspection rate of positive was 41.67％.Conclusions The detection of nucleic acid can make up the deficiency of serological test,and effectively reduce the leakage of transfusion-related infectious diseases,and ensuring the blood safety in this area.%目的 通过对浙南地区无偿献血者血清学和核酸筛查数据的回顾性分析,探讨核酸检测(Nucleic acid test,NAT)在降低输血相关传染性疾病中的作用.方法 选取2016年1月至2016年12月浙南地区无偿献血人员179 369人次,用酶联免疫吸附试验(ELISA)对血液传染性指标乙型肝炎病毒表面抗原(HBsAg)、丙型肝炎病毒抗体(抗-HCV)、人类免疫缺陷病毒抗原和抗体进行检测,同时采用罗氏、浩源核酸检测系统对HBV-DNA、HCV-RNA、HIV-RNA进行6或8人份混样三项目联合检测,统计分析血清学三项无反应性标本核酸检测(NAT)结果.结果 共检出259例ELISA-/NAT+样本,其中HBV-DNA+ 255例,HCV-RNA+5例,1例HBV-DNA重HCV-RNA+,阳性率为0.14％;罗氏核酸检测分析系统混检阳性率为1.40％,拆分阳性率为60.72％;浩源核酸检测分析系统混检阳性率为1.63％,拆分阳性率为41.67％.结论 核酸检测能弥补血清学检测的不足,有效降低输血相关传染性疾病的漏检,保障了地区的血液安全.