HPLC法测定心肾安颗粒中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量

摘要

Objective: To establish a method for determination of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in Xinshenan granules. Methods: Diamonsil C18 column (250mm × 4.6mm, 5 μm) was used with a mobile phase consisting of acetonitrile-water (16 : 84). The detection wavelength was 320 nm with a lfow rate of 1.0 mL·min-1. Column temperature was 35℃. Results:The 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside had a good linear relationship within the range of 3.7674 – 25.1160 μg·mL-1 (r = 1). The average recovery was 99.80%, RSD was 1.47% (n = 9).Conclusion:This method is simple, accurate and high sensitive, which can be used for content determination of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside inXinshenan granules.%目的:建立心肾安颗粒中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的测定方法.方法:色谱柱:Diamonsil C18柱(250 mm×4.6 mm,5μm);流动相:乙腈-水(16:84);检测波长:320 nm;流速:1.0 mL·min-1;进样量:10μL;柱温:35℃.结果:2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在3.7674~25.1160μg·mL-1范围内与峰面积呈良好的线性关系(r=1);2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的平均回收率为99.80%;RSD为1.47%(n=9).结论:本方法操作简单,专属性强,灵敏度高,可用于心肾安颗粒中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量测定.