Objective To evaluate the changes of serum amino acid level during 75 g OGTT and their relationship with insulin resistance in type 2 diabetes. Methods A total of 3 6 hospitalized patients with type 2 diabetes were enrolled in this study. Blood samples were collected before and 2 hours after glucose load during OGTT. Serum amino acids were measured by targeted LC-MS/MS. Results Val [(278. 32±57. 30)vs (245. 10±51. 65)μmol/L],Leu[(229. 45±42. 29)vs (184. 10±47. 19)μmol/L],Ile [(107. 03±36. 24)vs (85. 72±37. 39)μmol/L],Tyr[(66. 50±12. 17)vs (55. 50±13. 04)μmol/L]and Phe[(81. 00 ± 20. 49 )vs (69. 57 ± 17. 35 )μmol/L]significantly decreased after 75 g glucose load. Correlation analysis showed that the changes of Val,Ile and Phe after glucose load were positively associated with fasting insulin and HOMA-IR. Conclusion Serum amino acid levels significantly decrease after 75 g glucose load. Additionally,the higher level of fasting insulin and HOMA-IR are,the lower level of Val,Ile and Phe will be after glucose load.%目的：研究T2DM患者在行75 g OGTT过程中血清氨基酸水平的变化及其与IR的关系。方法选取36例于我院内分泌科住院的T2DM患者。收集行75 g OGTT过程中0 h及2 h血清样本。用液质联用技术（LC-MS／MS）检测血清氨基酸水平。结果与OGTT 0 h比较，OGTT 2 h缬氨酸[Val，（278．32±57．30）vs （245．10±51．65）μmol／L]、亮氨酸[Leu，（229．45±42．29）vs （184．10±47．19）μmol／L]、异亮氨酸[Ile，（107．03±36．24）vs （85．72±37．39）μmol／L]、酪氨酸[Tyr，（66．50±12．17）vs （55．50±13．04）μmol／L]、苯丙氨酸[Phe，（81．00±20．49）vs （69．57±17．35）μmol／L]水平下降（P＜0．001）。Pearson相关分析显示，Val差、Ile差、Phe差与FIns、HOMA-IR呈正相关。结论葡萄糖负荷后，血清氨基酸水平下降。FIns越高，HOMA-IR越大，葡萄糖负荷后Val、Ile、Phe下降越明显。
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