自体骨髓干细胞移植治疗糖尿病足溃疡的疗效及其与血清bFGF水平关系的研究

摘要

Objective To probe curative effect of autologous bone marrow stem cell transplantation on diabetic foot and its association with serum level of basic fibroblast growth factor (bFGF). Methods 79 diabetic foot cases caused by chronic lower limb ischemia were divided into regular treated group (group A: 40 cases, 80 affected limbs) and autologous bone marrow stem cell transplantation treated group ( group B: 39 cases, 78 affected limbs). And the curative effect as well as serum level of bFGF was evaluated after six-month treatment. Results ① After one-week treatment, there was statistically significant with limb pain, cold feeling of affected limbs, skin temperature, serum level of bFGF among group B compared with pretreatment (P＜0.05). There was correlation between serum level of bFGF and limb pain, cold feeling of affected limbs and skin temperature (P＜0.05). However, there was no statistically significance in the serum level of bFGF comparing one-month, three-month, six-month treatment with pretreatment respectively (P＞0.05). ②There was statistical significance in serum level of bFGF, limb pain, cold feeling of affected limbs and skin temperature between group B and group A after one-week treatment (P＜0.05). ③ After three-month and six-month treatment, clinical parameters showed statistical difference compared with pretreatment in group B (P＜0.05). There was statistical significance in all clinical parameters between group B and group A after three-month and six-month treatment (P＜0.05). Conclusion The curative effect of diabetic foot caused by chronic lower limb ischemia could be improved by autologous bone marrow stem cell transplantation, which could not induce side effect due to long-term increasing of serum level of bFGF.%目的 探讨自体骨髓干细胞移植治疗糖尿病足的疗效及其与血清碱性成纤维细胞生长因子(bFGF)水平的关系.方法 选择79例因慢性下肢缺血引起糖尿病足溃疡患者,分为常规治疗组(A组)40例,共80条患肢,自体骨髓干细胞移植治疗组(B组)39例,共78条患肢.治疗6个月,观察各项指标,对疗效及其与血清bFGF水平关系进行评估.结果 ①B组治疗后1周肢体疼痛、患肢冷感、皮肤温度、血清bFGF水平与治疗前比较差异有统计学意义(P<0.05),血清bFGF水平与肢体疼痛、患肢冷感、皮肤温度之间存在相关性(P<0.05).治疗后1个月、3个月、6个月血清bFGF水平与治疗前比较差异无统计学意义(P>0.05).②B组治疗后1周肢体疼痛、患肢冷感、皮肤温度、血清bFGF水平与A组治疗后1周比较差异有统计学意义(P<0.05).③B组治疗后3个月、6个月各项临床观察指标与治疗前比较差异均有统计学意义(P<0.05).B组治疗后3个月、6个月各项临床观察指标与A组治疗后相同时间点比较差异均有统计学意义(P<0.05).结论 自体骨髓干细胞移植技术治疗慢性下肢缺血引起糖尿病足溃疡可以提高疗效,不会导致由于血清bFGF水平长期增高引起的不良反应.