牙合力增强对大鼠牙周组织IL-6 mRNA表达影响的研究

摘要

目的：检测不同牙合力状态下，大鼠牙周膜成纤维细胞和牙槽骨成骨细胞中IL-6 mRNA的动态表达，探讨IL-6在牙周组织改建过程中的分子机制。方法：选用Wistar大鼠建立正常牙合力、牙合力增强的动物模型，采用原位杂交的方法，观察成纤维细胞和成骨细胞中IL-6 mRNA表达的动态变化。结果：牙合力增强诱导成纤维细胞和成骨细胞中IL-6 mRNA表达较正常牙合力时明显增强。结论：牙合力增强，促使牙周组织中的成纤维细胞和成骨细胞产生IL-6 mRNA明显增多，且出现一定的规律性变化，提示IL－6在牙合力影响牙周组织改建的过程中起着重要的调节作用。%AIM: To study the expression of IL-6 mRNA in rat periodontal ligament cells (PDLC) and osteoblasts under different occlusal forces, in order to explain the molecular mechanism of periodontium remodeling. METHODS: A rat model of different occlusal force was established by extracting the left maxillary molars, so that the left mandibular molar area was used as the bite force lost model and the right mandibular molar area as the bite force increased model. The untreated rats' mandibular molar area used as normal bite force model. The prepared tissue specimens were processed to study the expression of IL-6 mRNA in PDLC and osteoblasts with in situ hybridization technique. RESULTS: In the group of increased occlusal force the expression of IL-6 mRNA in PDLC and osteoblasts enhanced apparently than the group of normal occlusal force. CONCLUSION: The result of the increased occlusal force enhanced the expression of IL-6 mRNA in PDLC and osteoblasts suggested that the IL-6 may play important roles in the process of periodontium remodeling initiated by different occlusal force.