目的:观察静电纺聚乳酸-乙醇酸·蛋壳膜蛋白(PLGA ·SEP)混合纤维膜在体外降解过程中结构、性能的变化.方法:采用静电纺丝技术制备PLGA·SEP混合纤维膜和PLGA纤维膜(对照).将两种纤维膜浸泡在37℃,pH =7.4的模拟体液(simulated body fluid solution,SBF)中进行降解实验,并分别于降解后2、4、6周检测两种电纺纤维膜降解前后的质量、断裂强度、表面形貌和热分解温度.结果:两种电纺纤维膜的质量、断裂强度均随降解时间的延长而逐渐降低,在降解前2周,下降速度较快,从2周到6周速度减慢；在降解2、4、6周各时间点PLGA ·SEP纤维膜的失重率、断裂强度降低率均高于PLGA纤维膜,两者有显著性差异(P＜0.05).两种纤维膜均在降解6周时,纤维吸水发生溶胀,纤维之间粘连,孔径变小,但是结构仍完整,没有出现纤维断裂现象.PLGA·SEP和PLGA电纺纤维膜降解前后均有一个吸热峰,并且随着降解时间的延长,热分解温度逐渐降低,其中PLGA纤维膜在降解各时间点的热分解温度均高于PLGA·SEP纤维膜.结论:PLGA·SEP纤维膜在SBF中降解6周时结构仍完整并保持一定力学强度,可作为一种具有发展潜力的可吸:收性引导组织再生(guided tissue regeneration,GTR)膜.%AIM: To study the structure and performance changes of electrospun polylactic-co-glycolic-acid (PLGA)/soluble eggshell (SEP) membrane protein nanofibers film degradation in vitro. METHODS: PLGA/SEP and PLGA nanofibers ( control) membranes were prepared by electro spinning technology. The membranes were immersed in simulated body fluid solution (SBF) for degradation. Before and after degradation for 2,4 and 6 weeks. The quality, fracture strength, surface morphology and thermal decomposition temperature of the two membranes were tested. RESULTS; The quality and fracture strength of the two membranes decreased with the extention of time and in the first two weeks decreased faster then slower in the following weeks. The weight loss and decreased percentage of fracture strength of PLGA/SEP membrane were significantly higher than those of PLGA membrane at all time points (P < 0. 05 ) . At the end of week 6, PLGA/SEP and PLGA nanofibers swelled and became adhesive, and the pore size was smaller. However, the structure of nanofibers was still complete and no breakage was identified. An endothermic peak was found in both membranes before and after degradation. The temperature of endothermic peak decreased withthe extension of degradation. CONCLUSION; The PLGA/SEP nanofiber membrane maintains certain mechanical strength and structure integrity after 6 weeks of degradation and can be used as a potential absorbable guided tissue regeneration membrane.
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