内毒素致多脏器功能障碍综合征绵羊模型的建立

摘要

目的 建立简单有效的内毒素(lipopolysaccharide,LPS)致多脏器功能障碍综合征(multiple orgendysfunction syndrome,MODS)绵羊模型,观察其血气、血常规和血清生化等的变化特点.方法 绵羊全麻后,3μg/kg和6μg/kg LPS分别于30 min和60 min内静脉泵入实验Ⅰ组和实验Ⅱ组.以不同时点测定血气、血常规和血清生化的变化.结果 LPS静注后,实验Ⅰ组和Ⅱ组绵羊的死亡率分别为33.3%和50%;两实验组3 h内氧合指数明显降低(P<0.05或P<0.01);不同时点天冬氨酸转氨酶(AST)、丙氨酸氨基转移酶(ALT)、总胆红素(TBIL)和肌酐(CRE)明显升高(P<0.05或P<0.01);白细胞(WBC)数目在2 h明显降低,之后明显升高(P<0.05或P<0.01).结论 两种剂量的LPS均能成功地建立绵羊MODS模型;血气、血常规和血清生化等均有明显的变化.%Objective To establish an simple and effective animal model of multiple organ dysfunction syndrome (MODS) induced by lipopolysaccharide (LPS) in sheep and observe the changes of blood gas, blood routine test and blood biochemistry. Methods Twelve sheep (6 males and 6 femals) were randomized into group 1 and group 2, six sheep in each group. After anesthesia, 3 μg/kg LPS was infused intravenously within 30 minutes in the group 1 and 6 μg/kg LPS within 60 minutes in the group 2. The blood gas, blood routine test and blood biochemistry were measured at different time points. Results Two sheep in the group 1 (33.3％) and three sheep in the group 2 (50.0％) died after the intravenous injection of LPS. The oxygenation index was significantly decreased ( P ＜ 0.05 or P ＜ 0. 01 ), aspartic transaminase ( AST), alanine aminotransferase ( ALT), total bilirubin (TBIL) and creatinine (CRE) were significantly increased (P ＜0. 05 or P ＜ 0. 01 ) at different time points. The number of leukocytes was significantly decreased within 2 hours after intravenous injection of LPS, then markedly increased (P ＜ 0. 05 or P ＜ 0. 01 ). Conclusions A sheep model of MODS has been successfully established by two doses of LPS. Significant changes of the indexes of blood gas, blood routine test and biochemistry can be seen in those model sheep.