无菌大鼠血液学及血液生化指标正常参考值的测定

摘要

目的 人工培育Wistar无菌大鼠,观测Fl和F2代无菌大鼠血液学及血液生化指标差异.方法 对清洁级Wistar大鼠进行子宫摘除,隔离器内剥离子宫壁获得大鼠胎仔,人工哺育为无菌大鼠.待其繁殖后,采用全自动血液生化分析仪、全血细胞计数仪测定F1和F2代无菌大鼠的血液学及血液生化指标.结果 不同代数无菌大鼠的血液学和血液生化指标存在显著差异,其中有差异的血液学指标包括RBC、HGB、HCT、MCV、MCHC、RDW、MPV、NE%、LYM%、EO%、BA%、NE#、LYM#、EO#、BA#(P＜0.05);有差异的血液生化指标包括ALT、ALB、LDH、CHO、TBIL、HDL-C、LDL-C、GLO、GLU、ALP、A/G、UA、P(P＜0.05).同时这些差异又因性别和年龄而不同.结论 代数、性别、年龄对无菌大鼠的血液学及血液生化指标有较大影响.%Objective To determine and compare the blood parameters and blood biochemical parameters in F1 and F2 germ-free rats. Methods Germ-free rats were obtained by uterectomy, and the offspring were fed with artificial milk by hand in the germfree isolator. The blood parameters and blood biochemical parameters were detected by automatic hematology analyzers. Results The germ-free rats showed significant differences in the values of RBC, HGB, HCT, MCV,MCHC, RDW, MPV, NE％, LYM％, EO％, BA％, NE#, LYM #, EO#, BA # ( P ＜ 0.05 ) and ALT, ALB, LDH, CHO, TBIL,H DL-C, LDL-C,GLO,GLU,ALP,A/G, UA,P(P ＜ 0.05 ). There are different blood values in different gender and age.Conclusion The gender and age may affect the blood parameters and blood biochemical parameters.