建立大鼠持续性肠内营养的输注模型和淋巴液引流方法

摘要

目的 建立大鼠持续性肠内营养的输注模型和淋巴液引流的方法.方法 大鼠行胃造口后游离硅胶管至背部,通过swivel管连接到微量输液泵.5d持续匀速的输入肠内营养后开腹并游离肠淋巴干,大鼠在肠道缺血60 min再灌注120 min同时潜行插入硅胶管收集淋巴液.结果 第一次胃造口术后大鼠持续肠内营养输注通畅,未出现死亡或并发症状.第二次手术时通过较简单的方法可以获取淋巴液,成功率较高,可达90%以上.结论 大鼠持续肠内营养输注模型的建立,为研究营养物质的作用搭建了一个平台;通过较简单的方法获取淋巴液可以为研究淋巴液的成分提供便利.%Objective To establish model of continuous infusion of enteral nutrition and a method for obtaining lymph in rats. Method After rats received gastrostomy , the silicone tube , which freed to post back, was connected to the micro infusion pump through the swivel. After 5 days of continuous enteral nutrition the rats were opened the abdominal cavity and freed the intestinal lymph trunk, then the rats was subjected to intestinal ischemia for 60 minutes and reperfusion for 120 minutes, meanwhile, stealth inserted the silicon tube to collect lymph. Result After the first operation, the continuous infusion of enteral nutrition was smooth in rats and there were no death or complications symptoms. The lymph fluid was obtained by a simpler method, which success rate was higher than 90％ in the second operation. Conclusion Model establishment of continuous infusion of enteral nutrition in rats could greatly facilitate to study the role of nutrients.The simple method to obtain the lymph provides greatly convenience for the study of lymph ingredients.