The supramolecular interaction of gemfibrozil with β-cyclodextrin (β-CD) was studied by spectrofluorimetry.The mechanism of the inclusion was discussed by spectrofluoremetry, infrared spectrum and 1H NMR spectrum.The results showed that a 1 : 1 (β-CD : gemfibrozil) complex was formed with an apparent association constant of 3。844×103 L·mol-1.Based on the enhancement of the fluorscent intensity of gemfibrozil,a spectrofluorimetric method for the determination of gemfibrozil in bulk aqueous solution in the presence of β-CD was developed. The limear range was 3.30ng·mL-1-6.00μg·mL-1 with the detection limit of 0.980 ng·mL-1.There was no interference from the excipients normally used in tablet composition and the serum main compositions. The proposed method was then successfully applied to the determination of gemfibrozil in capsules and serum.
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