Direct Electrochemistry of Cytochrome c on EDTA-ZrO2 Organic-inorganic Hybrid Film Modified Electrodes

摘要

A composite film of ethylenediamine tetraacetic acid (EDTA)-ZrO2 organic-inorganic hybrid was prepared based on the chelation between Zr(Ⅳ) and EDTA. The direct electrochemical behavior of cytochrome c (cyt. c) at the hybrid film modified glassy carbon electrodes was investigated. The immobilized EDTA can promote the redox of heme in horse heart cyt. c which gives rise to a pair of reversible redox peaks with a formal potential of 40 mV (vs. SCE). The peak current increased linearly with the increase of cyt. c concentration in the range of 1.6 × 10-6_the electron transfer of cyt. c. The impediment capability of metal ions depends on their coordination capability with EDTA and their valence number.