Amperometric Sensor Based on Neutral Red-Doped Silica Nanoparticles Coupled with Microdialysis for the Measurement of Glutamate in the Rat Striatum

摘要

Amperometric sensor based on neutral red-doped silica (NRSiO2) nanoparticles (NPs) was fabricated and coupled with a microdialysis sampling system for the detection of glutamate (Glu) in the rat striatum. The NRSiO2 NPs [about (45±3) nm] were prepared with water-in-oil (W/O) microemulsion method, and characterized by transmission electron microscope (TEM) technique. The neutral red (NR) doped in silica network could maintain its high electroactivity and behave as an excellent electron mediator for electrocatalysis of hydrogen dioxide. Furthermore,the silica surface could prevent the leakage of NR, hence, the stability of biosensor was improved. The novel Glu biosensor showed a linear range from 5.0×10-7 to 1.5×10-4 mol/L, with a detection limit of 2.0×10-7 mol/L(S/N=3).