Nucleic Acid Biosensor for Detection of Human Immunodeficiency Virus Using Aquabis(1,10-phenanthroline)copper (Ⅱ) Perchlorate as Electrochemical Indicator

摘要

The electrochemical behavior of aquabis(1,10-phenanthroline)copper(Ⅱ) perchlorate[Cu(H2O)(phen)2]·2ClO4,where phen= 1,10-phenanthroline, on binding to DNA at a glassy carbon electrode (GCE) and in solution, was described. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) results showed that[Cu(H2O)(phen)2]2+.had excellent electrochemical activity on the GCE with a couple of quasi-reversible redox peaks. The interaction mode between[Cu(H2O)(phen)2]2+ and double-strand DNA (dsDNA) was identified to be intercalative binding. An electrochemical DNA biosensor was developed with covalent immobilization of human immunodeficiency virus (HIV) probe for single-strand DNA (ssDNA) on the modified GCE. Numerous factors affecting the probe immobilization, target hybridization, and indicator binding reactions were optimized to maximize the sensitivity and speed of the assay. With this approach, a sequence of the HIV could be quantified over the range from 7.8×10-9 to 3.1×10-7 mol·L-1 with a linear correlation of γ=0.9987 and a detection limit of 1.3×10-9 mol·L-1.