Department of Biotechnology;
South China University of Technology;
Guangzhou 510640;
China;
Department of Biotechnology;
South China University of Technology;
Guangzhou 510640;
Chinahe enantioselective esterification of racemic 1-trimethylsilylethanol with acids catalyzed by lipase in organic solvent was successfully performed. The influence of some factors on the reaction was investigated. Among the four lipases explored;
Candida rugosa lipase (CRL) showed the highest activity and enantioselectivity. Octanoic acid was the best acyl donor among the eleven acids studied and n-hexane was the most suitable medium for the reaction. The optimum shaking rate and temperature were found to be 150r.min-1 and 20℃to 30℃;
respectively. The enantiomeric excess of the remaining (5)-(-)-1-trimethylsilylethanol was 93% when substrate conversion was 53% upon incubation of the reaction mixture at 30℃;
150r.min-1 for 12 h.;
脂肪酶; 催化剂; 有机溶剂; 三甲硅烷基; 生产技术; 酰基;