Effect of HCMV IE1 Protein on Cytokines Secretion and Apoptosis of Macrophages

摘要

Objective:To investigate the effect of human cytomegalovirus(HCMV)IE1 protein on the secretory activity and apoptosis of macrophages. Methods:The eukaryotic expression vector pEGFP-C1/IE1 was used to transfect THP-1-macrophages.48 h after transfection,the expression and localization of GFP or GFP-IE1 was observed under fluorescent microscope.The levels of IL-1β and TNF-α in the culture media were examined by ELISA,and the mRNA expression of them was analyzed by RT-PCR.Cell undergoing apoptosis were determined by flow cytometry using the propidium iodide(PI) staining method.The data were analyzed by SPSS13.0. Results:As observed under fluorescent microscope,the expressions of GFP-IE1 and GFP by plasmid pEGFP-C1/IE1 or pEGFP-C1 in THP-1-macrophages could be found in nuclei or whole cells. Conclusion:As demonstrated by RT-PCR and ELISA,mRNA and protein expressions of IL-1β and TNF-α and promotes apoptosis in THP-1-macrophages.