Inhibitory Effects of 5-Aza-2'-Deoxycytidine and Trichostatin A in Combination with p53-Expressing Adenovirus on Human Laryngocarcinoma Cells

摘要

Objective:To investigate the effects of 5-Aza-2'-deoxycytidine (5-Aza-Cdr) and trichostatin A (TSA) combined with p53-expressing adenovirus (Ad-p53) on Hep-2 cell line in vivo and in vitro,in order to explore its possibility in biological treatment of laryngocarcinoma.Methods:Effects of 5-Aza-Cdr and TSA in combination with Ad-p53 on Hep-2 cell line in vivo were determined by Cell Counting Kit-8 (CCK-8) assay.The effect of drug combination was calculated by Jin's formula.Effects on the cell line in vitro were investigated by establishing the nude mice model.Results:5-Aza-Cdr and TSA showed inhibitory effects on the proliferation of Hep-2 cells in dose-and timedependent manner.Ad-p53 can inhibit the growth of Hep-2 cells in vivo and in vitro.However,the combination of epigenetic reagents (5-Aza-Cdr/TSA) and Ad-p53 was less effective than individual use of Ad-p53.5-Aza-Cdr and Adp53 inhibited the growth of transplanted tumors and reduced the volume of tumors,and the tumor volume of Ad-p53 group was significantly smaller than that of the control group (P＜0.05).Conclusion:Both epigenetic reagents (5-Aza-Cdr/TSA) and Ad-p53 can suppress cell proliferation on Hep-2 in vivo and in vitro and there may be some antagonistic mechanism between Ad-p53 and epigenetic reagents (5-Aza-Cdr/TSA).