MicroRNAs (miRNAs) are important regulators that play a key role in tumorigenesis and tumor progression.Transforming growth factor-β1 (TGF-β1) is involved in invasion and metastasis in many tumors.In this study,we investigated the microRNAs (miRNA) profiles altered by TGF-β1 in gastric cancer (GC) cells.Methods:We detected the expression profiles of miRNA by miRNA microarray and quantitative real-time polymerase chain reaction.Migration and invasion,wound-healing assay,prediction of miRNA targets,Western blot and qRT-PCR analysis were carried out to determine the role of one selected miRNA,namely miR-193 b,in affecting the biological behaviors of GC BGC823 cells.Results:Among 847 human miRNAs in the microarray,three miRNAs (miR-27a,miR-29b-1 and miR-194) were up-regulated and three (miR-574-3p,miR-193b and miR-130b) were down-regulated in BGC823 cells treated with TGF-β1 compared with control,miR-193b suppressed the invasion and metastasis of GC cells in vivo and in vitro,and down-regulated urokinase-type plasminogen activator (uPA) protein in GC cells.Conclusions:TGF-β1 altered miRNA expression profile in BGC823 cells.Among the altered miRNAs,TGF-β1 induced the down-regulation of miR-193b,which inhibited cell invasion and metastasis in vivo and in vitro,and down-regulated uPA protein in GC cells.
展开▼
