Relevances of microfilament and microtubule to the adhesion properties of the HCC onto the Collagen IV/ Laminin coated surface

摘要

The adhesion properties of both hepatocytes and hepatocellular carcinoma cells (HCCs) onto the Coll IV/laminin coated surface were measured by means of micropipette aspiration technique. Further,relevances of microfilament and microtubule systems to the adhesion properties of the HCCs on Coll IV/laminin coated dishes were investigated by using cytoskeletal agents colchicine and cytochalasin D. The results showed: cytochalasin D, which inhibits microfilament polymerization, had great inhibitory effect on adherence of both kinds of cells to Coll IV/ Laminin substratum (about 70%～90%). At the same time, HCCs extension obviously decreased and focal contacts almost vanished after being treated with cytochalasin D, in contrast, there was no significant effect on the formation of extention of hepatocytes. Colchicine, which inhibits microtubular polymerization, had different effects on both cells: Compared with untreated groups, the adhesion forces of HCC cells decreased and those of hepatocytes increased. These data suggested that, in these tumor cells, microfilament system are crucial for adherence, and abnormal cytoskeletons of tumor cells may be basis of their abnormal adhesion properties.