小麦长链非编码RNA的预测及功能分析

摘要

There is a large portion of transcribed DNA,which does not code for a functional protein.These long non-coding RNAs(lncRNA)appear to have epigenetic regulatory function in animals.While epigenetic gene regulation is also an essential mechanism in plants,relatively little is known about the presence or function of lncRNAs in plants.To explore the.function of lncRNA in plants,we have developed a computational pipeline for identified potential lncRNAs based wheat full length eDNA(t-eDNA)sequences,and there are 231 lncRNAs identified from 6162 wheat fl-cDNAs.Meanwhile,two novel miRNAs are identified from these lncRNAs,which indicate that wheat lncRNAs would play their roles by acting as precursors for small RNA molecules.And by sequence analysis,there are three conservative motifs present enrichment in lncRNAs,which shows that they may interact with other protein or DNA with these motifs.These findings are useful for exploration of lncRNAs function mechanism in plant.%生物体有部分基因被转录成RNA,但是不编码相应蛋白质,称为长链非编码RNA(IncRNA).它们参与基因的表观调控,这一过程对动物、植物的生长发育都有重要作用,但是,目前植物中发现和研究的IncRNA较少.为了研究lncRNA在植物中的功能,本研究建立了基于小麦全长cDNA的IncRNA识别程序.从6162条小麦全长cDNA中发现了231条IncRNAs,并从中鉴定出两个新miRNAs,这表明IncRNAs可以通过形成miRNAs前体基因形成其功能.此外,通过序列富集分析,我们从小麦IncRNAs中鉴定出三个保守的调控元件,结果显示小麦IncRNAs可能通过和其它蛋白质或DNA等分子作用,进而参与小麦生长、发育等过程的调控,这些结果对进一步研究植物体内的IncRNA的功能和作用机制具有重要意义.