三碘甲状腺原氨酸及格列本脲对缺血再灌注损伤后未成熟心肌细胞凋亡的影响

摘要

目的 通过三碘甲状腺原氨酸(T3)对缺血再灌注损伤后未成熟心肌细胞凋亡的影响,探讨其作用机制.方法 培养原代乳鼠未成熟心肌细胞,通过在缺氧复氧前给予T3或(和)格列本脲处理,用TUNEL检测法检测正常组、缺氧复氧处理组以及在缺氧复氧前给予T3或(和)格列本脲干预后心肌细胞凋亡情况.结果 缺氧复氧前短期给予T3可减少心肌细胞凋亡,而这种保护作用可被格列本脲阻断.结论 T3可以对抗缺血再灌注对心肌细胞损伤的能力,而对心肌细胞凋亡这种保护作用与KATP通道相关.%Purpose To investigate the effects of triiodothyronine (T3) on the apoptosis of immature cadiocytes while given ischemical reperfusion injury. Methods TUNEL was used to detect the apoptosis of immature cadiocytes in normal group, anoxia-reoxygenation group and T3/glibenclamide was administered before anoxia-reoxygenation group. Results The apoptosis of cadiocytes was reduced while given T3 shortly before anoxia-reoxygenation. This protection could be blocked by glibenclamide. Conclusion Giving T3 before anoxia-reoxygenation would protect immature cadiocytes against ischemical reperfusion injury. These effects were associated with the KATP channel blocker.