反刍家畜9种蠕虫卵显微特征的比较分析与数字化描述

摘要

The present study was undertaken to compare and analyze the microscopic characteristics of helminth eggs of ruminants,such as cattle and sheep,and realize the digital description of helminth egg images.Nine kinds of identified helminth were selected for obtaining abundant eggs from cloaca in this study.To establish helminth egg images database,the mature eggs were selected and photographed by biological microscope.The sizes and shapes of all helminth eggs were compared and the Pseudo-Jacobi-Fourier Moments were used for feature extraction and digital description.The result showed that,the length and width of different helminth eggs all have significant differences,the invariant moment values of the same kind of eggs were almost the same,but different kinds of eggs revealed large difference in the invariant moment values.The reconstruction images of the helminth eggs is satisfied,and they are much similar to original images,hence,the PJFM's is suitable to extract the key feature point of eggs images.Therefore,our study can provide important technologic basis for digitization description and intellegent identification of helminth eggs.%为了比较分析牛、羊等反刍家畜常见蠕虫卵的显微特征和实现虫卵图像的数字化描述,选取已鉴定好的9种常见蠕虫,采用显微解剖方法由其泄殖腔取出大量虫卵制备压片,并借助光学显微镜认真观察,选择轮廓清晰、接近成熟的虫卵进行拍照,建立原始图像库.比较各虫卵的大小和形态特征,并用变形雅可比(p=4,q=3)-傅立叶矩(PJFM's)对原始虫卵图像进行特征提取和数字化描述.结果发现,不同种虫卵的长径、宽径均有显著性或极显著性差异,同一种虫卵特征的不变矩值基本相等,而不同种虫卵的不变矩值则相差很大.虫卵重建图像试验结果较好,其形态特征基本和原图像一致,说明PJFM's(p=4,q=3)矩能够很好地提取虫卵图像的关键特征点,可成功描述蠕虫卵图像.因此,该研究结果为实现虫卵的数字化描述和智能识别奠定了重要的技术基础.