枸杞多糖对卵形鲳鲹生长性能、抗氧化能力及血清免疫、生化指标的影响

摘要

本试验旨在研究枸杞多糖对卵形鲳鲹 (Trachinotus ovatus) 生长性能、抗氧化能力及血清免疫、生化指标的影响.选取平均体质量为 (7.45±0.06) g的卵形鲳鲹幼鱼360尾, 随机分为6组, 每组3个重复, 每个重复20尾, 分别饲喂枸杞多糖含量为0 (对照) 、0.05％、0.10％、0.20％、0.40％和0.80％的6种饲料.试验期8周.结果显示:1) 各组末质量、增重率和特定生长率差异不显著 (P>0.05) .2) 0.10％和0.20％组肝脏超氧化物歧化酶活性显著高于对照组 (P<0.05) , 丙二醛含量显著低于对照组 (P<0.05) .0.10％、0.20％、0.40％和0.80％组肝脏过氧化氢酶活性显著高于对照组 (P<0.05) .0.10％组肝脏谷胱甘肽过氧化物酶活性和总抗氧化能力显著高于对照组 (P<0.05) .3) 0.10％组血清补体3和补体4含量均显著高于对照组 (P<0.05) .0.10％和0.20％组血清溶菌酶活性显著高于对照组 (P<0.05) .4) 0.10％和0.20％组血清葡萄糖含量显著低于0.05％和对照组 (P<0.05) .0.10％、0.20％和0.40％组血清胆固醇含量显著低于0.05％和对照组 (P<0.05) .0.10％组血清甘油三酯含量显著低于0.05％和对照组 (P<0.05) .0.10％和0.20％组血清高密度脂蛋白含量显著高于对照组 (P<0.05) , 低密度脂蛋白含量显著低于对照组 (P<0.05) .综上所述, 枸杞多糖可以显著提高卵形鲳鲹的抗氧化能力和免疫力, 而促生长的作用不显著.卵形鲳鲹幼鱼饲料中枸杞多糖的适宜添加量为0.10％.%To study the effects of Lycium barbarum polysaccharide (LBP) on growth performance, antioxidant capacity, serum immune and biochemical indexes of juvenile golden pompano (Trachinotus ovatus) , six diets were prepared with the addition of 0 (control) , 0.05％, 0.10％, 0.20％, 0.40％ and 0.80％ LBP, respectively.A total of 360 fish with an average body weight of (7.45±0.06) g were assigned randomly into six groups with three replicates per group and 20 fish per replicate.The experiment lasted for eight weeks.The results showed as follows:1) the final weight, weight gain rate and specific growth rate were not significantly affected among groups (P>0.05) .2) The superoxide dismutase activity in liver of 0.10％ and 0.20％ groups was significantly higher than that of control group (P<0.05) , while the content of malondialdehyde in liver was significantly lower than that of control group (P<0.05) .The catalase activity in liver of 0.10％, 0.20％, 0.40％ and 0.80％ groups was significantly higher than that of control group (P<0.05) .The glutathione peroxidase activity and total antioxidant capacity in liver of 0.10％ group were significantly higher than those of control group (P<0.05) .3) The contents of complement 3 and complement 4 in serum of 0.10％ group were significantly higher than those of control group (P<0.05) .The lysozyme activity in serum of 0.10％ and 0.20％groups was significantly higher than that of control group (P < 0.05) .4) The content of serum glucose of0.10％ and 0.20％ groups was significantly lower than that of 0.05％ group and control group (P<0.05) .The content of serum cholesterol of 0.10％, 0.20％ and 0.40％ groups was significantly lower than that of 0.05％group and control group (P<0.05) .The content of serum triglyceride of 0.10％ group was significantly lower than that of 0.05％ group and control group (P < 0.05) .The content of serum high density lipoprotein of0.10％ and 0.20％ groups was significantly higher than that of control group (P<0.05) , and low density lipoprotein content was significantly lower than that of control group (P<0.05) .In conclusion, dietary LBP can significantly improve antioxidant capacity and immunity of juvenile golden pompano, but not the growth.The suitable supplemental level of LBP in diets of juvenile golden pompano is 0.10％.