The poly (acrylic acid) functionalized NaYF4:Yb,Er nanoparticles were found to possess intrinsic peroxidase-like activity, which could catalyze oxidization of 3 ,3',5,5'-tetramethylbiphenyl (TMB) by H2O2 to produce a colour variation. Using TMB as substrate, we systematically studied the effect of a series of conditions, such as temperature and pH, on the catalytic activity of NaYF4 : Yb,Er nanoparticles. When combined with uricase, the proposed colorimetric method could be successfully used for the determination of uric acid. Under the optimum conditions, the determination of uric acid was in the range from 1. 0xl0-5 to 2.0x10 mol/L with the detection limit down to 5. 3 × 10-6 mol/L. Moreover, the developed method was applied to detect uric acid in serum samples and the results were in good agreement with those reported by the hospital.%聚丙烯酸修饰的NaYF4∶Yb,Er纳米粒子能够有效地催化H2O2氧化3,3',5,5'-四甲基联苯胺(TMB)产生显色反应,表现出良好的过氧化物模拟酶催化活性.本研究以TMB为模型底物,研究了催化条件(温度和pH值)对催化活性的影响.同时,结合尿酸在尿酸氧化酶作用下产生H2O2的原理,建立了比色法测定血样中尿酸含量的简便方法.在最优条件下,本方法的检测范围为1.0×10-5～2.0×10-4mol/L,检出限为5.3×10-6 mol/L,并对实际样品进行测定,测定结果与临床结果一致.
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