龙须菜芽诱导研究

摘要

试验以龙须菜茎段为外植体,研究不同消毒方法和不同培养基对龙须菜芽诱导的影响,建立龙须菜芽诱导体系。结果表明：最佳的外植体消毒方法是先用70%的酒精消毒15s,再用0.1%的升汞消毒6min,效果最好；诱导培养基以MS+6-BA1.0mg/L+NAA0.05mg/L效果最好。%The effect of different sterilization methods, hormone kinds and concentrations on bud induction of Sechium edule Swarte were studied in this test. The results indicated that the most effective sterilization treatment was 70% alcohol 15 s, 0.1%HgCl2 6 min; The optimum initial culture medium is MS+6-BA 1.0 mg/L+NAA 0.05 mg/L.