维吾尔族饲鸽者肺患者细胞因子水平变化及临床意义研究

摘要

Objective To explore the pathogensis of pigeon - breeder - lung - disease( PBL) by determining the changes of bronchoalveolar lavage fluid(BALF),serum interleukin(IL) - 2,IL - 10,tumor necrosis factor( TNF - α). Methods From 2012 to 2013,65 Uygur pigeon - feeders diagnosed as PBL(study group)by department of respiration,the Xinjiang Uygur Autonomous Region People's Hospital,120 healthy pigeon - feeders without PBL(negative control group),120 healthy subjects who did not feed pigeons(normal control group)were enrolled in this study. BALF and the levels of IL - 2,IL- 10,TNF - α were detected by enzyme linked immunosorbent assay(ELISA). Results In the BALF and serum,IL - 2, TNF - α were higher in study group than in groups negative control,normal control(P < 0. 05),higher in group negative con-trol than in normal control(P < 0. 05). And IL - 10 levels were lower in groups study,negative control than in normal control group(P < 0. 05),there was no significant difference between groups study,negative control(P > 0. 05). Conclusion In Uygur PBL patients,the BALF and serum IL - 10 decreases,IL - 2,TNF - α increase. IL - 2,TNF - α may be involved in the occurrence and development of PBL.%目的：探讨维吾尔族饲鸽者肺（PBL）患者支气管肺泡灌洗液（ BALF）与血清中白介素（IL）-2、IL -10、肿瘤坏死因子-α（TNF -α）水平的变化及其在 PBL 发病机制中的作用。方法选取2012-2013年在新疆（喀什、和田）地区至少饲养鸽子1年以上的维吾尔族受试者，将新疆维吾尔自治区人民医院呼吸科确诊为 PBL 的受试者65例作为病例组，未患 PBL 的受试者120例作为阴性对照组；选取同期体检健康的未饲鸽的维吾尔族受试者120例为正常对照组。采用酶联免疫吸附试验（ELISA）检测3组受试者 BALF 与血清中 IL -2、IL -10、TNF -α水平。结果病例组 BALF 与血清中 IL -2、TNF -α的表达水平均较阴性对照组、正常对照组升高，阴性对照组较正常对照组亦升高（P <0.05）。病例组、阴性对照组 BALF 与血清中 IL -10的表达水平较正常对照组均降低（P <0.05），而病例组与阴性对照组间差异无统计学意义（P >0.05）。结论维吾尔族 PBL 患者 BALF 与血清中 IL -10水平降低，IL -2、TNF -α水平升高；IL -2、TNF -α可能参与了 PBL 的发生发展。