Cloning and Sequence Analysis of a Full-length cDNA Encoding Light-harvesting Complexes of Photosystem II in Phyllostachys edulis

摘要

The light-harvesting chlorophyll a/b-protein complex plays an important role in photosynthesis of plants. A full-length cDNA of light-harvesting chlorophyll a/b (cab) gene was cloned from the first strand of Moso (Phyllostachys edulis) cDNA through RT-PCR and RACE methods, named as cab- PhE10 (cab gene 10 from Ph. edulis). The length of cab- PhE10 (GenBank accession number: EU118754) is 1 151 bp, which contains an open reading frame encoding 283 amino acids from 81st to 932nd position. The bioinformatics analysis indicated that the protein encoded by cab-PhE10 had a chlorophll a/b binding domain (83rd -247th position), two protein kinase C-phosphorylation sites, three N- myristoylation sites and a yia A/B double helix domain.The amino acid sequence of cab-PhE10 showed high similarity with the cab genes of Oryza sativa, Zea mays, Hordeum vulgare, and Vitis vinifera, more than 80%, respectively, which indicated that cab-PhE10 gene belongs to lhcb5 gene family.