RAS、CD68和CD34在乳腺癌组织中的表达及其临床意义

摘要

Objective To investigate the expression of active RAS,CD68 and CD34 in breast cancer and their clinical signifi⁃cance. Methods Immunohistochemistry was used to detect the expression of RAS, CD68, CD34 in breast cancer tissues. The correla⁃tion of the level of RAS with TNM staging, lymph node metastasis, HER⁃2, ER and PR, tumor size and so on was analyzed. The corre⁃lation between RAS and CD68, CD34 expression in breast cancer tissues were investigated. Results Of the 65 cases of breast cancer tissues, 43 cases were positive for RAS and 63 cases were positive for CD68, the median values of CD68 positive cells were 12, and the median value of microvessle density( MVD) was 49. RAS expression level was significantly positively related with length to diameter of tumor and HER⁃2(r=0�342,P=0�005;r=0�334,P=0�006).CD68 positive cells level was significantly positively related with length to diameter of tumor, TNM staging and HER⁃2(r=0�303,P=0�014;r=0�358,P=0�003;r=0�325,P=0�008).RAS level was significantly positively related with CD68 positive cells level( r=0�277,P=0�026) . MVD level was positively related with CD68 posi⁃tive cells level( r=0�330, P=0�007) . Conclusion The horizontal of RAS and the CD68 positive cells in breast cancer are signifi⁃cantly related with HER⁃2,tumor size,TNM stage. RAS may affect the process of MVD generation promoted by macrophages, and its mechanism still needs to be further studied.%目的探讨RAS、CD68和CD34在乳腺癌中的表达情况及其临床意义。方法收集2010年1月至2012年9月泗洪县人民医院普外科手术切除的乳腺癌患者肿瘤组织蜡块65例，采用免疫组化染色法检测乳腺癌组织标本中活化的RAS、CD68和CD34的表达水平。分析RAS表达水平与TNM分期、淋巴结转移、HER⁃2、ER、PR、肿块大小等临床病理特征的关系及其与CD68、CD34表达的相关性。结果65例乳腺癌标本中，43例RAS阳性，63例CD68阳性，中位CD68阳性细胞数为12个，中位微血管密度（MVD）为49个。 RAS表达与肿瘤直径、HER⁃2呈显著正相关（r＝0�342，P＝0�005；r＝0�334，P＝0�006）。 CD68阳性细胞水平与肿瘤直径、TNM 分期、HER⁃2呈显著正相关（ r＝0�303， P＝0�014； r＝0�358， P＝0�003；r＝0�325，P＝0�008）。 RAS水平与CD68阳性细胞水平呈显著正相关（ r＝0�277，P＝0�026）。 MVD水平与CD68阳性细胞水平呈显著正相关（ r＝0�330，P＝0�007）。结论乳腺癌中RAS、CD68阳性细胞水平与HER⁃2、肿瘤直径、TNM分期明显相关。 RAS可能影响了肿瘤相关巨噬细胞促进MVD生成的过程，其机制仍需进一步研究。