Fluorescence Study on the Interaction between Naproxen and Yeast DNA

摘要

The interaction between naproxen and yeast DNA was studied using fluorescence spectrometry and ultra-violet (UV) spectrometry. The addition of yeast dsDNA to naproxen solution resulted in a strong fluorescence quenching. Both the Stern-Volmer and the Scatchard plots of the fluorescence quenching showed a curve with two slopes and a threshold DNA concentration of about 100 μtmol·L-1. The interaction between naproxen and the dsDNA was found to be a groove binding mode by UV spectra, the influence of ion strength and I- quenching effect. This mode was independent on DNA concentration. However, the increase in DNA concentration changed the binding constant K of naproxen to yeast dsDNA and the number of binding sites in DNA base pairs (n).