A Furostanol Saponin from the Cytotoxic Fraction of Tupistra chinensis Rhizomes

摘要

A furostanol saponin was obtained from the n-butanol fraction of methanol extract from Tupistra chinensis rhizomes, a folk medicine of Shennongjia Forest District of Hubei Province. Its structure was determined as 3-O-β-D-glucopyranosyl-(25S)-22-O-methyl-5β-furost-1β, 3β, 5β, 22α, 26-pentaol-26-O-β-D-glucopyranoside (1) on the basis of chemical and spectroscopic evidences. The n-butanol fraction displayed marked inhibitory activity in vitro towards HeLa and HL-60 human tumor cell lines by MTT method.