Highly-efficient photosensitizer based on AIEgen-decorated porphyrin for protein photocleaving

摘要

An AIEgen decorated porphyrin(TPETPyP)was easily obtained through a one-step reaction.The bulky TPE in TPETPyP greatly impeded the intermolecularπ-πstacking of the porphyrin core,which significantly suppressed aggregation-caused quenching(ACQ)effect of TPETPyP in aqueous solution.The four pyridinium salts formed in TPETPyP also render the whole molecule water solubility,which eliminated its aggregation.TPETPyP exhibited 1O2 quantum yield as high as 0.85 in PBS.Moreover,it also showed high binding affinity to proteins,the major biotarget of 1O2.The high 1O2 quantum yield plus the great binding ability of TPETPyP toward proteins makes it a highly-efficient protein photocleaving agent.Protein electrophoresis experiments demonstrated that TPETPyP can photocleave BSA upon visible light irradiation,indicating that TPETPyP can act as a promising photosensitizer(PS)in PDT.The work here will provide a facile strategy to utilize AIEgens modified traditional PSs for photodynamic therapy(PDT).