Studies of Ce(Ⅲ)-ALC-F Interacting with Herring Sperm DNA by Electrochemical, Fluorimetric and UV-spectrophotometric Method

摘要

Ce(Ⅲ)-ALC-F^- complex can react with hsDNA to form an electrochemically non-active supermolecular complex Ce(Ⅲ)-ALC-F^--DNA in the buffer solution of (CH2)6N4(pH=4.9),which results in the decrease of the peak current of Ce(Ⅲ)-ALC-F^-.This method can be applied to determine DNA concentration.In addition,by using fluorimetric and UV-spectrophotometric methods with studies of denatured DNA and the effect of NaC1 solution,it is also found that the binding mode is intercalation.