NMR identification of anti-influenza lead compound targeting at PAc subunit of H5N1 polymerase

摘要

PAC subunit from avian influenza (H5N 1) viral RNA polymerase was used in this work as a target in the screening for antiinfluenza agents from licorice-derived compounds.As a result,18β-glycyrrhetinic acid was suggested to be PAC ligand by flexible docking,and was then confirmed by relaxation-edited NMR.The result of ApG primer extension assay indicated that this PAC ligand can inhibit the polymerase activity,and thus may potentially be valuable as anti-influenza lead compound.This work validated the possibility of screening polymerase inhibitors by using PAc as a target,and provided a starting point for the further discovery of new anti-influenza drugs.