Although it is well known that water is essential for biological function,it has been a challenge to determine how water behaves near biomacromolecular interfaces,and what role water plays in influencing the dynamics of the biochemical machinery.By adopting a vibrational labeling strategy coupled with ultrafast two-dimensional infrared(2D-IR) spectroscopy,it has recently become possible to study hydration dynamics,site specifically at the surface of proteins and model membranes.We review our recent progress in measuring hydration dynamics in contexts ranging from small-molecule solutes to biomacromolecules in dilute,viscous,and crowded environments.
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