2型糖尿病、冠状动脉硬化性心脏病与血清脂联素、网膜素、内脏脂肪素的相关性探索

摘要

Objective To investigate the level changes of serum adiponectin, omentin, and visfatin in patients with type 2 diabetes mellitus (T2DM) or coronary heart disease (CHD).Methods There were 30 health people as control group, 34 cases of type 2 diabetes mellitus as T2DM group, and 41 cases of coronary heart disease as CHD group. A double antibody sandwich enzyme-linked immunosorbent assay (ELISA) method was applied to detect the levels of serum adiponectin, omentin, and visfatin of the patients. Automatic blood biochemical analyzer was used for the detection of fasting serum glucose (FPG), total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C). Chemiluminescence was used to detect fasting serum insulin (FINS), and homeostasis model assessment of insulin resistance (HOMA-IR) was applied to assess the insulin resistance index.Results The levels of serum adiponectin and omentin of T2DM group and CHD group were significantly lower than that of the control group (F=34.99, 100.65,q=7.60~21.68, P<0.01). The levels of serum visfatin of T2DM group and CHD group were obviously higher than that of the control group (F=21.28,q=9.25, 8.30,P<0.01). The differences of indexes between T2DM group and CHD group were no statistically significant (P>0.05). The serum adiponectin, omentin, and visfatin in each group all had correlation with HOMA-IR (P<0.01).Conclusion Serum adiponectin, omentin and visfatin are closely correlated with the occurrence and development of T2DM and CHD.%目的：探讨血清脂联素、网膜素、内脏脂肪素在2型糖尿病(T2DM)患者及冠状动脉硬化性心脏病(CHD)患者中的变化。方法健康体检者30例为对照组,2型糖尿病患者34例为T2DM组,冠状动脉硬化性心脏病患者41例为CHD组。采用双抗体夹心法酶联免疫吸附试验(ELISA)检测各组患者血清脂联素、网膜素和内脏脂肪素含量；采用全自动血生化分析仪分别检测各组患者空腹血清葡萄糖(FPG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)含量,采用化学发光法检测各组患者空腹血清胰岛素(FINS)含量,采用稳态模型评估法(HOMA-IR)计算胰岛素抵抗指数。结果 T2DM组、CHD组血清脂联素、网膜素水平均明显低于对照组(F=34.99,100.65,q=7.60~21.68,P<0.01)；T2DM组、CHD组血清内脏脂肪素水平明显高于对照组(F=21.28,q=9.25,8.30,P<0.01), T2DM组与CHD组以上指标比较差异无统计学意义(P>0.05)。各组血清脂联素、网膜素、内脏脂肪素与HOMA-IR均有相关性(P<0.01)。结论血清脂联素、网膜素、内脏脂肪素与2型糖尿病、冠状动脉硬化性心脏病的发生发展密切相关。