北京万寿菊灰霉病病原菌分离鉴定

摘要

对北京四海色素万寿菊制种温室发生的疑似灰霉病调查发现,万寿菊植株发生叶片水渍状枯斑、萎蔫等,严重的甚至整株枯萎死亡,造成了重大损失.为明确其病原,对病原菌进行了分离、培养和快速致病性测定,并对分离的菌株进行DNA提取和基因序列分析.结合生物学特征和基于基因序列的分子进化树分析,鉴定该病原菌为灰葡萄孢(Botrytis cinerea).%Suspected grey mold disease was investigated in Sihaisesu greenhouse of marigold seed production bases in Beijing,where symptoms of hygrophanous spot and wilts on marigold and even dead plant with heavy loss were found.The pathogen was isolated and cultured, DNA extraction of the isolated strains were conducted, and the sequences of g3pdh、hsp60 and rbp2 were analyzed.Based on biological characteristics and molecular evolutionary tree of particular genes,the pathogen was identified as Botrytis cinerea.