刺齿凤尾蕨抗肿瘤活性成分5F的含量测定

摘要

目的:建立测定刺齿凤尾蕨中抗肿瘤成分5F含量的方法,并测定其不同部位中5F的含量.方法:采用高效液相色谱法.色谱柱为Hypersil C18(250mm×4.6 mm,5 μm),流动相为甲醇-1％醋酸水溶液(梯度洗脱),流速为1.0 mL·min-1,检测波长为242nim,柱温为30℃.结果:5F检测浓度在6～96 μg· mL-1范围内与峰面积积分值呈良好线性关系(r＝0.998 1);低、中、高浓度对照品加入量对应的平均加样回收率分别为103.33％、100.84％、100.39％,RSD分别为5.86％、3.05％、1.02％(n均为6).5F在刺齿凤尾蕨叶中含量最高,茎中含量其次,根中含量最低.结论:本方法稳定、快速且杂质干扰小,可用于刺齿凤尾蕨中5F的含量测定.%OBJECTIVE: To establish an method for content determination of antitumor active component 5F from Pteris dispar, and to determine the content of 5F in different parts. METHODS: HPLC method was adopted. The separation was performed on Hypersil C18(250 mm×4.6 mm,5 μm)column with mobile phase consisted of methanol-1% acetic acid (gradient elution) at the flow rate of 1.0 mL·rnin-1. UV detection wavelength was set at 242 nm, and the column temperature was maintained at 30 ℃. RESULTS: The linear range of 5F was 6~96 μg·mL-1(r=0.998 1). The average recoveries were 103.33%, 100.84% and 100.39% , and RSDs were 5.86% , 3.05% and 1.02% (n=6).The content of 5F in the leaves of P. dispar was the highest, followed by the steam and root. CONCLUSION: The methods is stable,rapid and less interference from impurity, and can be used for the content determination of 5F in P. dispar.