Complete nitrogen removal was achieved through integrating anammox and autotrophic denitrification in an UASB reactor.The total nitrogen(TN)removal rate increased stepwise from 0.46 to 0.94 kg-N/(m3·d),with an effluent TN concentration of below 3.0 mg-N/L achieved.The process is relatively insensitive to the nitrite to ammonium ratio,achieving complete nitrogen removal when their ratio in the influent varied in the range of 1.35-1.55.The added S0 quantity in the system could be utilized to adjust the competition between autotrophic denitrifiers and anammox bacteria.High-throughput sequencing technology indicated that Candidatus_Kuenenia and Thiobacillus were the functional strains for anammox and autotrphic denitrification process,respectively,in the studied reactor.This result provides a theoretical and technical basis for the large-scale application of anaerobic ammonium oxidation process.
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