Immobilization of Agaricus Bisporus Laccase on Ceramic-Chitosan Composite Support and Their Properties:Potential for Oily Wastewater Treatment

摘要

Laccase was immobilized on the ceramic-chitosan composite support by using glutaraldehyde as the cross-linking reagent. The immobilization conditions and characterization of the immobilized enzyme were investigated. The immobili-zation of laccase was successfully realized when 3.0 mL of 1.25 mg/mL of laccase at a pH value of 4.0 reacted with 0.15 g of ceramic-chitosan composite support (CCCS) at 4℃ for 24 h. The immobilized enzyme exhibited a maximum activity at pH 3.0. The optimal temperatures for immobilized enzyme were 25℃ and 50℃. TheKm value of immobilized laccase for ABTS was 66.64 μmol/L at a pH value of 3.0 at 25℃. Compared with free laccase, the thermal, operating and storage sta-bility of immobilized laccase was improved after the immobilization.