不明原因不孕症患者子宫内膜血管内皮生长因子的表达与体内激素的相关性研究

摘要

Objective To study the correlation of vascular endothelial growth factor (VEGF) and hormone in patients with unexplained infertility. Methods 18 patients with unexplained infertility treated in our hospital from January 2010 to January 2014 were selected as observation group,while 18 healthy people were selected as control group.At the se-cretory phase,the expression of VEGF of endometrium of the two groups was detected based on the immunohistochemi-cal streptomycin avidin-SP method.At the 3-5 day of menstrual cycle and secretory phase,the venous blood of two groups was drawn.Based on the isotope radio-immuno-assay,the level of FSH,LH,E2,T,P and PRL was detected.The positive rate of VEGF and hormone level of two groups was compared while the correlation between VEGF and hor-mone was analyzed. Results The expression of VEGF of observation group was lower than that of control group (P<0.05). At secretory phase,the level of E2 and P of observation group was lower than that of control group (P<0.05);the level of PRL of observation group was higher than that of control group (P<0.05);FSH,LH and T of two groups had no significant different (P>0.05).For observation group,the expression of VEGF and the level of FSH,LH,T and PRL at menstrual cy-cle,and the level of E2 and P at secretory phase had no correlation (P>0.05). Conclusion The unexplained infertility may be correlated with VEGF of endometrium;at secretory phase,the reduction of estrogen and progestin may cause the pa-tient’s infertility.However,VEGF and hormone content at different phases is not significantly correlated.%目的：探讨不明原因不孕症患者子宫内膜血管内皮生长因子(VEGF)的表达情况以及与体内激素之间的关系。方法入选2010年1月~2014年1月于本院就诊的不明原因不孕症患者18例作为研究对象，同时选择同期健康对照组18例，两组受试者均进行分泌期子宫内膜获取，应用免疫组织化学链霉素抗生物素蛋白-过氧化酶连接法(SP法)进行VEGF在子宫内膜中的表达测定，两组受试者均在月经3~5 d和分泌期子宫内膜获取日进行静脉血抽取，应用同位素放射免疫法进行促卵泡成熟激素(FSH)、黄体生成激素(LH)、雌二醇(E2)、睾酮(T)、孕酮(P)和催乳素(PRL)水平检测。比较两组的VEGF阳性率和激素水平，同时对VEGF和激素进行相关性分析。结果观察组VEGF的表达较对照组明显降低，差异有统计学意义(P<0.05)。观察组分泌期的E2和P水平较对照组明显降低，差异有统计学意义(P<0.05)；观察组PRL水平较对照组明显升高，差异有统计学意义(P<0.05)；两组的FSH、LH、T水平差异无统计学意义(P>0.05)。观察组VEGF表达与月经周期第3天FSH、LH、T、PRL及分泌期的E2和P之间无明显相关性(P>0.05)。结论不明原因不孕症可能与子宫内膜VEGF有相关性，分泌期雌激素和孕激素水平下降可能为不孕病因，但VEGF与不同时期激素水平无明显相关性。