低分子肝素钠对体外培养胰腺癌细胞刺激的人淋巴内皮细胞增殖的影响

摘要

Objective To discuss whether the low molecular weight heparin (LMWH) can affect HLEC cell proliferation induced by the supematant of PANC-1. Methods To evaluate the anti-lymphangiogenic effect of LMWH on human lymphatic endothelial cell (HLEC) proliferation by an in vitro assay. Results The supematant of pancreatic cancer cell (PANC-1) could stimulate HLEC cell proliferation. There was significant deviation in HLEC proliferation between conventional culture (MTT: OD = 0.26 ± 0.02) and cultured with the supernatant of PANC-1 (MTT: OD =0.41 ±0.04). LOw concentration LMWH did not affect the proliferation of PANC-1 and HLEC cells, but suppressed HLEC cell proliferation induced by the supematant of PANC-1. Conclusion This study showes that PANC-1 supernatant plays an important role in promoting HLEC cell proliferation. Low concentra-tion LMWH is effective in inhibiting HLEC cell proliferation induced by the supematant of PANC-1.%目的 探讨低分子肝素钠对胰腺癌细胞培养上清液诱导的人淋巴内皮细胞增殖的影响.方法 胰腺癌细胞培养上清液同人淋巴内皮细胞混合培养,经噻唑蓝(MTT)测比较低分子肝素钠作用于胰腺癌细胞株诱导的人淋巴内皮细胞增殖差异.结果 人淋巴内皮细胞同胰腺癌细胞上清液共同培养后,其MTT检测吸光度(A)值达到(0.41±0.04),与单纯内皮淋巴细胞A值(0.26±0.02)比较,差异有统计学意义(P<0.01).同时低浓度低分子肝素钠能抑制胰腺癌细胞株上清液刺激的人淋巴内皮细胞增殖.结论 体外培养的胰腺癌细胞株上清液能刺激人淋巴内皮细胞增殖.低分子肝素钠可以抑制胰腺癌细胞株培养上清液诱导人淋巴内皮细胞的增殖作用.