目的：建立人工牛黄的鉴别方法；建立用高效液相色谱法测定羌活、白芷中欧前胡素、异欧前胡素的含量测定方法。方法采用薄层色谱法鉴别人工牛黄，以胆酸、猪去氧胆酸、人工牛黄为对照物，点样量分别为10μL；采用C18色谱柱，乙腈-水（55∶45）为流动相；检测波长300nm。结果在紫外光灯（365nm）下检视样品斑点分离度较好，Rf值适宜；欧前胡素、异欧前胡素混合标准溶液进样量分别在0.029328～0.87984μg和0.024192～0.72576μg范围内，与峰面积呈良好的线性关系，欧前胡素平均回收率为99.51%，RDS=1.42%；异欧前胡素平均回收率为98.65%，RDS=2.66%。结论方法专属性强、耐用性高，准确度好，操作简便。%Objective To establish the method for identification of artificial bezoar; To establish a HPLC notopterygium, angelica imperatorin, isoimperatorin method for the determination. Methods Identification by TLC artificial bezoar to bile acids, hyodeoxycholic, artificial bezoar as a control substance, point injection volume was 10μL; The C18 Column, Acetonitrile- water (55 ︰ 45) as the mobile phase, detection wavelength 300nm. Results UV light (365nm), under review sample spots better separation, Rf value suitable; imperatorin, isoimperatorin mixed standard solution injection quantities were reported in 0.029 328-0.879 84μg and 0.024 192-0.725 76μg range, and the peak area was a good linear relationship, imperatorin the average recovery was 99.51%, RDS=1.42%;isoimperatorin the average recovery was 98.65%, RDS=2.66%. Conclusion This method is specific, high durability, good accuracy,and easy to operate.
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