白藜芦醇对C3H10T1/2间质干细胞成骨分化及CXCL12、EGFR和CCL2基因表达的影响

摘要

Objective To study the effects of resveratrol on early osteoblast differentiation and mRNA expression of CXCL12, EGFR and CCL2 of C3H10T1/2 mesenchymal stem cells. Methods The C3H10T1/2 cell proliferation was detected using CCK-8 after different concentrations of resveratrol. The early osteoblast differentiation was identified by alkaline phosphatase (ALP) staining. The mRNA expression of CXCL12, EGFR and CCL2 was detected using real time quantitative RT-PCR. Results The resveratrol less than 20 μmol/L concentrations had no effect on C3H10T1/2 cell proliferation (P> 0.05), however, high concentrations of resveratrol (40, 80, 100 μmol/L) inhibited the C3H10T1/2 cell proliferation (P< 0.05). 20 μmol/L resveratrol enhanced rhBMP-2-induced ALP staining of C3H10T1/2 cell, but the gene expressions of CXCL12, EGFR and CCL2 were not changed (P>0.05). Conclusion Resveratrol can promote the rhBMP-2-induced osteogenic differentiation, but which may not act through regulating the mRNA expression of Cx-cl12, Egfr and Ccl2.%目的：探讨白藜芦醇对C3H10T1/2细胞成骨分化的作用及CXCL12、EGFR和CCL2基因表达的影响。方法采用CCK-8法检测不同浓度白藜芦醇对C3H10T1/2细胞增殖影响，碱性磷酸酶染色鉴定早期成骨分化，实时荧光定量PCR法检测CXCL12、EGFR和CCL2基因的表达。结果白藜芦醇在20μmol/L浓度对C3H10T1/2细胞增殖没有影响(P>0.05)，随着浓度增加，40、80、100μmol/L的白藜芦醇明显抑制细胞增殖(P<0.05)。20μmol/L白藜芦醇能增强重组人骨形成蛋白2(rhBMP-2)诱导C3H10T1/2的碱性磷酸酶染色。白藜芦醇对C3H10T1/2细胞CXCL12、EGFR、CCL2基因表达没有明显影响(P>0.05)。结论白藜芦醇能促进rhBMP-2诱导成骨分化。促成骨分化作用可能不是通过调控CXCL12、EGFR和CCL2基因的表达来实现。