天麻最细粉对帕金森病大鼠脑内神经递质及环磷酸腺苷/环磷酸鸟苷的调节作用

摘要

Objective To investigate the effects of superfine powder and common powder of Gastrodia elata on neuro-transmitters and cyclic adenosine monophosphate (cAMP)/cyclic guanosine monophosphate (cGMP) regulating in rats with Parkinson's disease (PD). Methods Model of PD rats were established with injection of 6-hydroxydopamine (6-O-HDA) into the substantia nigra of right side brain. The mouse were divided into the normal group, model group, Gastro-dia elata common powder group (1.8 g/kg), and superfine powder group (1.8 g/kg). After 3 weeks of admimistration, the learning and memory was determined by water maze test in all the groups. The levels of dopamine (DA), noradrenaline (NE), 5-hydroxytryptamine (5-HT) were assayed by high performance liquid chromatography-electrochemical detection technique. cAMP and cGMP were assayed by euzymelinked immunosorbent assay. Results After treatment with su-perfine and common powder of Gastrodia elata, the escape latency was significantly shorter than the PD model rats (P<0.05), the levels of DA, NE and 5-HT of rats were significantly higher than those in the PD model rats (P<0.05), and serum cAMP significantly improved, cGMP signifi-cantly reduced, cAMP/cGMP significantly improved (P<0.05). The impacts on pharmacological actions were more significant in superfine powder group than in common powder group (P < 0.05). Conclusion Both the superfine and common powder of Gastrodia elata can improve the learning and memory ability, recover the levels of DA, NE, 5-HT and cAMP/cGMP in PD rats. Fur-thermore, the results indicate that the Gastrodia elata after superfine grinding can enhance the curative effect, which provides experimental references for the clinical study of superfine powder of Gastrodia elata.%目的：观察天麻最细粉和普通粉对帕金森病(PD)大鼠脑内神经递质及血清中环磷酸腺苷(cAMP)/环磷酸鸟苷(cGMP)的调节作用,对比最细粉和普通粉的疗效差别。方法采用脑内定位注射6-羟基多巴胺(6-OHDA)建立PD大鼠模型。设立正常组、模型组、天麻普通粉组(1.8 g/kg)和天麻最细粉组(1.8 g/kg)。每天灌胃给药1次,连续给药3周后,应用水迷宫实验检测大鼠学习记忆能力；采用高效液相色谱-电化学检测法测定大鼠脑组织中神经递质---多巴胺(DA)、去甲肾上腺素(NE)和5-羟色胺(5-HT)的含量变化；酶联免疫法测定大鼠血清中cAMP和cGMP的含量及cAMP/cGMP的比值。结果经过天麻普通粉和天麻最细粉的治疗后,两组大鼠的逃避潜伏期明显缩短(P<0.05),且天麻最细粉组对大鼠学习记忆能力的改善作用优于普通粉组(P<0.05)；天麻治疗后大鼠脑组织的DA、NE、5-HT水平均显著高于模型组(P<0.05),且天麻最细粉组的改善作用优于天麻普通粉组(P<0.05)；天麻治疗后大鼠血清中的cAMP显著上升(P<0.05),cGMP显著降低(P<0.05),导致cAMP/cGMP的比值显著升高(P<0.05),趋于正常,而且天麻最细粉组的疗效明显优于天麻普通粉组(P<0.05)。结论经过天麻普通粉和天麻最细粉的治疗后,PD大鼠的学习记忆能力得到改善,提高了PD大鼠脑组织中降低了的DA、NE、5-HT水平,调节了血清中cAMP/cGMP平衡,且天麻最细粉的疗效优于普通粉,为天麻最细粉的临床使用提供了科学依据。