Objective To provide an effective method for the quality of cleaning and disinfection of rigid endoscope based on compared results of two detection methods between the hard endoscope cleaning and disinfection quality. Methods We selected 120 pieces rigid endoscopes which were sterilized and determined qualified by visual method in our department from October 2014 to October 2015. At the same time, the outer surface and the internal cavity of the rigid endoscope was carried on sampling detection using ATP bioluminescence detection and strip method, respectively. Results The surface positive rate of rigid endoscope sterilized by ATP fluorescence detection method was larger than that was sterilized by strip method, but there was no significant difference between the two groups (P>0.05). The internal cavity positive rate and whole positive rate were significantly greater than that of strip method (P<0.05). The RLU value of internal cavity of rigid endoscope was significantly higher than that of instrument surface value. Conclusion A scientific and reasonable testing methods can quickly and objectively evaluate the quality of cleaning and disinfection. ATP fluorescence detection method is simple, rapid and practical, which can improve the quality of hard endoscope cleaning and disinfection.%目的 对比两种检测方法 在集中式管理硬式内镜清洗消毒质量的检测效果,为硬式内镜清洗消毒质量提供有效的检测方法.方法选取2014年10月~2015年10月在我科室进行清洗消毒,目测法判定合格的硬式内镜120件,同时使用ATP生物荧光检测与杰力试纸法对硬式内镜的外表面及内部腔道进行采样检测.结果 ATP荧光检测法在检验硬式内镜器械表面阳性率大于杰力试纸法,但是组间比较差异无统计学意义(P>0.05).在检验硬式内镜内部腔道时阳性率及总阳性率均明显大于杰力试纸法,差异有统计学意义(P<0.05).硬式内镜内部腔道RLU值明显高于器械表面值.结论 选择科学合理的检测方法,能快速、客观地评价清洗消毒质量.ATP荧光检测法简便、快速、实用性强,可提高硬式内镜清洗消毒质量.
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